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  1. Perennial grasslands, including prairie and pasture, have declined with tremendous environmental and social costs. This decline reflects unequal policy support for grasslands and managed grazing compared to row crops. To create a resource for community partners and decision-makers, we reviewed and analyzed the policy tools and implementation capacity that supports and constrains grasslands and managed grazing in the U.S. Upper Midwest. Risk reduction subsidies for corn and soybeans far outpace the support for pasture. Some states lost their statewide grazing specialist when the federal Grazing Lands Conservation Initiative lapsed. The United States Department of Agriculture, Natural Resources Conservation Service support for lands with prescribed grazing practices declined after 2005 but remained relatively steady 2010–2020. These results reveal the policy disadvantage for grasslands and managed grazing in comparison with row crop agriculture for milk and meat production. Grassland and grazing policies have an important nexus with water quality, biodiversity, carbon and outdoor recreation policy. Socially just transitions to well-managed, grazed grasslands require equity-oriented interventions that support community needs. We synthesized recommendations for national and state policy that farmers and other grazing professionals assert would support perennial grasslands and grazing, including changes in insurance, conservation programs, supply chains, land access, and fair labor. These policies would provide critical support for grass-based agriculture and prairies that we hope will help build soil, retain nutrients, reduce flooding and enhance biodiversity while providing healthy food, jobs, and communities. 
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  2. Dominant forms of agricultural production in the U.S. Upper Midwest are undermining human health and well being. Restoring critical ecosystem functions to agriculture is key to stabilizing climate, reducing flooding, cleaning water, and enhancing biodiversity. We used simulation models to compare ecosystem functions (food-energy production, nutrient retention, and water infiltration) provided by vegetation associated with continuous corn, corn-soybean rotation, and perennial grassland producing feed for dairy livestock. Compared to continuous corn, most ecosystem functions dramatically improved in the perennial grassland system (nitrate leaching reduced ~90%, phosphorus loss reduced ~88%, drainage increased ~25%, evapotranspiration reduced ~29%), which will translate to improved ecosystem services. Our results emphasize the need to incentivize multiple ecosystem services when managing agricultural landscapes. 
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  5. At two sites in the North Central USA (Michigan (KBS) and Wisconsin (ARL)), we evaluated the effect of N fertilization on the yield and quality of five perennial bioenergy feedstock cropping systems: (1) switchgrass (Panicum virgatum L.), (2) giant miscanthus (Miscanthus × giganteus), (3) a native grass mixture (5 species), (4) an early successional field (volunteer herbaceous species), and (5) a restored prairie (18 species). In a randomized complete block design with 5 replicates and 2 split plots, N was applied at 0 and 56 kg ha−1 to split plots for each cropping system from 2010 to 2016. No yield response to N was detected in switchgrass at either location in any year. Giant miscanthus exhibited a positive yield response to N at both sites (11% at KBS and 83% at ARL). Nitrogen fertilizer addition significantly reduced glucose (KBS 12.9 and 13.8 g kg−1 year−1, ARL 11.2 and 9.7 g kg−1 year−1) in the native grass mix and restored prairie systems respectively. Nitrogen fertilizer also reduced xylose at KBS in the switchgrasss, native grass mix, and restored prairie (4.9, 7.5, and 5.0 g kg−1 year−1). At ARL, N fertilization reduced xylose levels in switchgrass, giant miscanthus, and restored prairie (7.4, 6.8, and 6.2 g kg−1 year−1) and increased xylose levels in the early successional system (5.0 g kg−1 year−1). 
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